Application
Immunocytochemistry of DISC1 in HeLa cells with DISC1 antibody at 5 μg/mL.Application
Western blot analysis of DISC1 in SK-N-SH cell lysate with DISC1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of DISC1 in HeLa cells with DISC1 antibody at 20 μg/mL.| Product Name | DISC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DISC1 Antibody: SCZD9, C1orf136, KIAA0457, Disrupted in schizophrenia 1 protein |
| Product description | DISC1 Antibody: Disrupted in schizophrenia 1 (DISC1) is a candidate gene for susceptibility to schizophrenia. It was discovered through chromosomal analysis of a large Scottish family whose members exhibited schizophrenia and related psychiatric disorders. Through yeast two-hybrid screening, it was discovered that DISC1 interacts with many members of the centrosome and cytoskeletal system including MAP1A and Nudel. More recently, DISC1 has been found to regulate the transport of a complex containing Nudel, the lissencephaly-1 (LIS1) protein, and 14-3-3epsilon from neuronal cell bodies to the axons by the action of the microtubule-dependent directed motor protein kinesin-1, also known as KIF5A. Decreased expression of DISC1 in neurons caused an accelerated rate of neuronal integration, resulting in aberrant morphological development, suggesting that DISC1 plays a role in dendritic development and synapse formation.1) Millar JK, Wilson-Annan JC, Anderson S, et al. Disruption of two novel genes by a translocation co-segregating with schizophrenia. Hum. Mol. Genet. 2000; 9:1415-23. |
| Immunogen | DISC1 antibody was raised against an 18 amino acid synthetic peptide from near the center of human DISC1. The immunogen is located within amino acids 350 - 400 of DISC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| DISC1 antibody can be used for detection of DISC1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DISC1 |
|---|---|
| Alternative Names | DISC1 Antibody: SCZD9 C1orf136 KIAA0457 Disrupted in schizophrenia 1 protein |
| Molecular Weight(MW) | Predicted: 72, 92, 94 kDa Observed: 94 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | At least four isoforms of DISC1 are known to exist; this antibody will detect the three longest isoforms. |
Application
Immunocytochemistry of DISC1 in HeLa cells with DISC1 antibody at 5 μg/mL.
Application
Western blot analysis of DISC1 in SK-N-SH cell lysate with DISC1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of DISC1 in HeLa cells with DISC1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | DISC1 antibody can be used for detection of DISC1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DISC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DISC1 Antibody is supplied in PBS containing 0.02% sodium azide. |
