Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Product Name | DOT1L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DOT1L, DOT1, KIAA1814, KMT4, SCZD9, C1orf136 |
| Product description | DOT1L is a histone methyltransferase. It methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones.1) Steger, D.J., (2008) Mol. Cell. Biol. 28 (8), 2825-2839. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DOT1L. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DOT1L antibody can be used for detection of DOT1L by ELISA at 1:62500. DOT1L antibody can be used for detection of DOT1L by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DISC1 |
|---|---|
| Alternative Names | DOT1L DOT1 KIAA1814 KMT4 SCZD9 C1orf136 |
| Molecular Weight(MW) | 165 kDa |
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | DOT1L antibody can be used for detection of DOT1L by ELISA at 1:62500. DOT1L antibody can be used for detection of DOT1L by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DOT1L antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
