Application
Western blot analysis of Disp2 in rat brain tissue lysate with Disp2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Disp2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Disp2 Antibody: DISPB, HsT16908, DISPB, KIAA1742, Protein dispatched homolog 2 |
| Product description | Disp2 Antibody: Disp2 is the second of three known homologs of the D. melanogaster protein Dispatched. It is a multi-transmembrane protein containing two PTCH/DISP domains and is thought to be involved in the release of lipid-anchored Hedgehog from producing cells. Hedgehog is a major player in signaling pathways during embryogenesis, tissue regeneration, and carcinogenesis and the Disp proteins have been implicated in these pathways. Recently, it has been shown that Disp2 is translationally regulated by the microRNA miR-214 in zebrafish. Expression of this miRNA decreased Disp2 promoter activity in vitro and its overexpression in zebrafish resulted in a phenotype identical to that observed by Disp2 mutants. At least two isoforms of Disp2 are known to exist. This Disp2 antibody is predicted to not cross-react with Disp1 or Disp3.1) Katoh Y and Katoh M. Identification and characterization of DISP3 gene in silico. Int. J. Oncol.2005; 26:551-6. |
| Immunogen | Disp2 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human Disp2. The immunogen is located within amino acids 1210 - 1260 of Disp2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Disp2 antibody can be used for the detection of Disp2 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DISP2 |
|---|---|
| Alternative Names | Disp2 Antibody: DISPB HsT16908 DISPB KIAA1742 Protein dispatched homolog 2 |
Application
Western blot analysis of Disp2 in rat brain tissue lysate with Disp2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | Disp2 antibody can be used for the detection of Disp2 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Disp2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Disp2 Antibody is supplied in PBS containing 0.02% sodium azide. |
