Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of DKK2 using DKK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DKK2 gene (Lane 2).Application
Antibody is used in Western blot to detect DKK2 in Jurkat cell lysate.| Product Name | DKK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dickkopf-related protein 2, Dickkopf-2, Dkk-2, hDkk-2, DKK2 |
| Product description | The 259-amino acid DKK2 protein, like DKK1, DKK3, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cys residues each. The second cys region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed expression of 4.0- and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15- to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos.1) Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). Brott, B.K., et al., Mol. Cell. Biol. 22(17):6100-6110 (2002). Krupnik, V.E., et al., Gene 238(2):301-313 (1999). |
| Immunogen | This DKK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-62 amino acids from the N-terminal region of human DKK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | DKK2 |
|---|---|
| Alternative Names | Dickkopf-related protein 2 Dickkopf-2 Dkk-2 hDkk-2 DKK2 |
| Molecular Weight(MW) | 28 kDa |
Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of DKK2 using DKK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DKK2 gene (Lane 2).
Application
Antibody is used in Western blot to detect DKK2 in Jurkat cell lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
