Application
Western blot analysis of HepG2 in 293 cell lysate with DLK1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of DLK1 in HepG2 cells with DLK1 antibody at 20 μg/ml.Application
Immunocytochemistry of DLK1 in HepG2 cells with DLK1 antibody at 2.5 μg/ml.| Product Name | DLK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DLK1 Antibody: DLK, Protein delta homolog 1, pG2, DLK-1 |
| Product description | DLK1 Antibody: The Delta-like 1 homolog (DLK1) is a transmembrane protein containing six epidermal growth factor repeats. It is involved in the differentiation of several cell types, including adipocytes and is also thought to be a tumor suppressor. The DLK1 gene is one of several imprinted genes located in a region on chromosome 14q32; certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). DLK1 is expressed from the paternal allele; a polymorphism within this gene has been associated with child and adolescent obesity.1) Smas CM and Sul HS. Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation. Cell 1993; 73:725-34. |
| Immunogen | DLK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DLK1. The immunogen is located within amino acids 260 - 310 of DLK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| DLK1 antibody can be used for detection of DLK1 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DLK1 |
|---|---|
| Alternative Names | DLK1 Antibody: DLK Protein delta homolog 1 pG2 DLK-1 |
| Molecular Weight(MW) | 42 kDa |
| Sequence Similarities | 2) Cat. No. 17-011 - Hep G2 Cell Slide |
| Tissue Specificity | At least four isoforms of DLK1 are known to exist. |
Application
Western blot analysis of HepG2 in 293 cell lysate with DLK1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of DLK1 in HepG2 cells with DLK1 antibody at 20 μg/ml.
Application
Immunocytochemistry of DLK1 in HepG2 cells with DLK1 antibody at 2.5 μg/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | DLK1 antibody can be used for detection of DLK1 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DLK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DLK1 Antibody is supplied in PBS containing 0.02% sodium azide. |
