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ELISA: (direct or indirect: 1:2,000-1:10,000). Flow Cytometry: (1:100-1:500). Immunohistochemistry: (paraffin sections (1:100-1:500)). Western blot: (1:2,000-1:10,000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.:
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