Application
Western blot analysis of DLK2 using rabbit polyclonal DLK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DLK2 gene.Application
Western blot analysis of DLK2 Antibody in HL-60 cell line lysates (35ug/lane)Application
DLK2 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DLK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein delta homolog 2, DLK-2, Epidermal growth factor-like protein 9, EGF-like protein 9, DLK2, EGFL9 |
| Product description | DLK2 (Delta like protein 2) affects adipogenesis of 3T3-L1 preadipocytes and mesenchymal C3H10T1/2 cells suggesting a role as a regulator of adipogenesis.1) Mural R.J., et.al., Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. |
| Immunogen | This DLK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-380 amino acids from the C-terminal region of human DLK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DLK2 |
|---|---|
| Alternative Names | Protein delta homolog 2 DLK-2 Epidermal growth factor-like protein 9 EGF-like protein 9 DLK2 EGFL9 |
| Molecular Weight(MW) | 41 kDa |
Application
Western blot analysis of DLK2 using rabbit polyclonal DLK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DLK2 gene.
Application
Western blot analysis of DLK2 Antibody in HL-60 cell line lysates (35ug/lane)
Application
DLK2 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
