| Product Name | DLL4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Delta-like Protein 4; Delta4 |
| Product description | The Notch ligand delta-like protein 4 (DLL4) is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch1 and Notch4. It is induced by VEGF as a negative feedback regulator and acts to prevent overexuberant angiogenic sprouting, pro-moting the timely formation of a well differentiated vascular network. DLL4-Notch1 signaling regulates the formation of appropriate numbers of tip cells to control vessel sprouting and branching in the mouse retina. |
| Immunogen | Recombinant human DLL4 (extracellular domain). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | DLL4 |
|---|---|
| Alternative Names | Delta-like Protein 4 Delta4 |
| Tissue Specificity | Recognizes human DLL4. |
| Entrez Gene | 54567 |
|---|---|
| Protein Accession | NP_061947.1 |
| Application Notes | ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 1 year after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives. |
