Transcription factors containing a homeodomain play an important role in the organogenesis of vertebrates. Otx3/Dmbx1 is a member of paired class homeodomain transcription factors. Otx3/Dmbx1 represses the Otx2-mediated transactivation in the developing brain by forming heterodimer with Otx2 on the P3C (TAATCCGATTA) sequence in vitro.1) Kimura, K., et al., (2005) FEBS Lett. 579 (13), 2926-2932.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse DMBX1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
DMBX1 antibody can be used for detection of DMBX1 by ELISA at 1:62500. DMBX1 antibody can be used for detection of DMBX1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human brain at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1303 - Human Brain Tissue Lysate
Application Notes
DMBX1 antibody can be used for detection of DMBX1 by ELISA at 1:62500. DMBX1 antibody can be used for detection of DMBX1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store DMBX1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.