Application
Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated PhalloiApplication
Western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).| Product Name | DMRTA2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Doublesex- and mab-3-related transcription factor A2, Doublesex- and mab-3-related transcription factor 5, DMRTA2, DMRT5 |
| Product description | DMRTA2 may be involved in sexual development.1) Ottolenghi, C., et al. Genomics 79(3):333-343(2002) |
| Immunogen | This DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouseZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DMRTA2 |
|---|---|
| Alternative Names | Doublesex- and mab-3-related transcription factor A2 Doublesex- and mab-3-related transcription factor 5 DMRTA2 DMRT5 |
| Molecular Weight(MW) | 53 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloi
Application
Western blot analysis in PC-3 cell line, mouse testis and zebra fish brain tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
