Application
Western blot analysis of lysed extracts from HT29 and HepG2 cells using DNA PKcs (Ab-2609).| Product Name | DNA PKcs Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HYRC, p350, DNAPK, DNPK1, HYRC1, XRCC7, DNA-PKcs, EPLG5, HTKL, LERK5, PRKD, PRKDC, P460 |
| Product description | The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties1) Chan DW, et al. (2002) Genes Dev. Sep 15; 16 (18): 2333-2338Ding Q, et al. (2003) Mol Cell Biol. Aug; 23 (16): 5836-5848Douglas P, et al. (2002) Biochem J. Nov 15; 368 (Pt 1): 243-251 |
| Immunogen | DNA PKcs (Ab-2609) antibody was raised against a peptide sequence around aa.2607~2611 (V-E-T-Q-A) derived from Human DNA-PK. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKDC |
|---|---|
| Alternative Names | HYRC p350 DNAPK DNPK1 HYRC1 XRCC7 DNA-PKcs EPLG5 HTKL LERK5 PRKD PRKDC P460 |
| Molecular Weight(MW) | 450 kDa |
| Tissue Specificity | This antibody detects endogenous level of total DNA PKcs protein. |
| Entrez Gene | 5591 |
|---|---|
| Protein Accession | NP_001075109.1 |
Application
Western blot analysis of lysed extracts from HT29 and HepG2 cells using DNA PKcs (Ab-2609).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
