Application
Immunohistochemistry staining of PRKDC in prostate tissue using PRKDC Antibody.| Product Name | PRKDC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKDC, DNA-PK, DNAPK, DNPK1, HYRC, HYRC1, p350, Scid, XRCC7, DNA-PK catalytic subunit, DNA-PKcs, p460, DNA-dependent protein kinase catalytic subunit |
| Product description | DNA dependent Protein Kinase (also called DNAPK, DNPK1, HYRC1, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC7 and p460) consists of the 460 kDa DNA PKcs and a heterodimeric regulatory complex comprised of p70 Ku and p80 Ku (Ku autoantigen). DNA PKcs is a nuclear protein serine/threonine kinase present in a wide variety of eukaryotic species. DNA PKcs phosphorylates transcription factors, Sp1, Oct-1, p53 and SV40 large T antigen. DNA PKcs is involved in repairing double stranded DNA breaks. At the onset of apoptosis, DNA PKcs is rapidly inactivated by cleavage of the catalytic subunit into smaller polypeptides. Proteolysis of DNA PKcs is inhibited by the cysteine protease inhibitors iodoacetamide and N-ethylmaleimide. Alternative splicing can occur for this protein to produce at least two isoforms. |
| Immunogen | PRKDC antibody was raised against aa 2604 - 2614 of PRKDC (Human). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| PRKDC antibody can be used in ELISA starting at 1:4000 - 1:16000, Western Blot starting at 1:500 - 1:3000, and immunohistochemistry starting at 10 - 20 μg/mL.: |
|
| Species Reactivity | ChimpanzeeHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKDC |
|---|---|
| Alternative Names | PRKDC DNA-PK DNAPK DNPK1 HYRC HYRC1 p350 Scid XRCC7 DNA-PK catalytic subunit DNA-PKcs p460 DNA-dependent protein kinase catalytic subunit |
| Tissue Specificity | pThr2609 |
Application
Immunohistochemistry staining of PRKDC in prostate tissue using PRKDC Antibody.| Application Notes | PRKDC antibody can be used in ELISA starting at 1:4000 - 1:16000, Western Blot starting at 1:500 - 1:3000, and immunohistochemistry starting at 10 - 20 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store PRKDC antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
