Application
Western blot analysis of DNAJA4 in human colon tissue lysate with DNAJA4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | DNAJA4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DnaJ homolog subfamily A member 4, MST104, MSTP104, PRO1472 |
| Product description | The DNAJA4 protein is the mammalian homolog of the chaperone HSP40 (1) and acts as a co-chaperone with the heat shock protein HSP70 (2). DNAJA4 is highly expressed in heart and testis, accounting for approximately 1% of total protein in mouse heart (2). DNAJA4 is also regulated by the sterol regulatory element binding transcription factor 2 (SREBF2) and plays a role in the synthesis of cholesterol (3).1) Ohtsuka K and Hata M. Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature. Cell Stress Chaperones 2000; 5:98-112. |
| Immunogen | DNAJA4 antibody was raised against a 15 amino acid peptide near the center of human DNAJA4. The immunogen is located within amino acids 210 - 260 of DNAJA4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DNAJA4 antibody can be used for detection of DNAJA4 by Western blot at 1 - 2 μg/ml.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DNAJA4 |
|---|---|
| Alternative Names | DnaJ homolog subfamily A member 4 MST104 MSTP104 PRO1472 |
| Molecular Weight(MW) | Predicted: 41, 44, 47 kDa Observed: 40 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (87%) |
| Tissue Specificity | DNAJA4 antibody is human, mouse, and rat reactive. At least three isoforms of DNAJA4 are known to exist; this antibody will detect all three isoforms. |
Application
Western blot analysis of DNAJA4 in human colon tissue lysate with DNAJA4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1320 - Human Colon Tissue Lysate |
|---|---|
| Application Notes | DNAJA4 antibody can be used for detection of DNAJA4 by Western blot at 1 - 2 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DNAJA4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | DNAJA4 antibody is supplied in PBS containing 0.02% sodium azide. |
