Application
Immunohistochemistry of DNAJC13 in human heart tissue with DNAJC13 antibody at 5 μg/mL.Application
Western blot analysis of DNAJC13 in human heart tissue lysate with DNAJC13 antibody at 1 μg/ml.| Product Name | DNAJC13 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNAJ homolog subfamily C member 13, receptor-mediated endocytosis 8, RME8 |
| Product description | DNAJC13, also known as receptor-mediated endocytosis 8 (RME8), is the human homolog to a DnaJ domain-containing protein originally identified in a screen for endocytic defects in C. elegans (1). It is thought to be a co-chaperone of Hsc70 which regulates protein conformation at membrane sites and plays a role in intracellular trafficking, co-localizing with markers of the endosomal system. Recent experiments have indicated that the DNAJC13 protein is involved in membrane trafficking through early endosomes but not through degradative organelles (2). DNAJC13 has been also been shown to regulate the intracellular trafficking of the epidermal growth factor receptor (3).1) Girard M, Poupon V, Blondeau F, et al. The DnaJ-domain protein RME-8 functions in endosomal trafficking. J. Biol. Chem. 2005; 280:40135-43. |
| Immunogen | DNAJC13 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DNAJC13. The immunogen is located within amino acids 1940 - 1990 of DNAJC13. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| DNAJC13 antibody can be used for detection of DNAJC13 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DNAJC13 |
|---|---|
| Alternative Names | DNAJ homolog subfamily C member 13 receptor-mediated endocytosis 8 RME8 |
| Molecular Weight(MW) | Predicted: 249 kDa Observed: 249 kDa |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
| Tissue Specificity | DNAJC13 antibody is human and mouse reactive. At least two siforms of DNAJC13 are known to exist; this antibody will detect both isoforms. DNAJC13 antibody is predicted to not cross-react with other DNAJC family members. |
Application
Immunohistochemistry of DNAJC13 in human heart tissue with DNAJC13 antibody at 5 μg/mL.
Application
Western blot analysis of DNAJC13 in human heart tissue lysate with DNAJC13 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | DNAJC13 antibody can be used for detection of DNAJC13 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DNAJC13 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | DNAJC13 antibody is supplied in PBS containing 0.02% sodium azide. |
