DnaJ homolog subfamily C member 24, CSL-type zinc finger-containing protein 3, Diphthamide biosynthesis protein 4, DNAJC24, DPH4, ZCSL3
Product description
Diphthamide is a unique posttranslationally modified
histidine found only in translation elongation factor-2 (EEF2; MIM
130610). This modification is conserved from archaebacteria to
humans and serves as the target for ADP-ribosylation and
inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas
exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of
diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied
by OMIM].1) Liu, S., et al. Mol. Cell. Biol. 24(21):9487-9497(2004)
Immunogen
This DNAJC24 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-81 amino acids from the Central region of human DNAJC24.
Western blot analysis in HL-60 cell line lysates (35ug/lane).
Application Notes
For WB starting dilution is: 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.