Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
DNAJC6 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in K562(lane 1),HL-60 cell line(lane 2) and mouse brain tissue(lane 3) lysates (35ug/lane).| Product Name | DNAJC6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Putative tyrosine-protein phosphatase auxilin, DnaJ homolog subfamily C member 6, DNAJC6, KIAA0473 |
| Product description | DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain1) Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010) |
| Immunogen | This DNAJC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 254-281 amino acids from the Central region of human DNAJC6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DNAJC6 |
|---|---|
| Alternative Names | Putative tyrosine-protein phosphatase auxilin DnaJ homolog subfamily C member 6 DNAJC6 KIAA0473 |
| Molecular Weight(MW) | 100 kDa |
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
DNAJC6 Antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in K562(lane 1),HL-60 cell line(lane 2) and mouse brain tissue(lane 3) lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
