Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | DNAJC9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DnaJ homolog subfamily C member 9, DnaJ protein SB73, DNAJC9 |
| Product description | DNAJC9 is upregulated at both mRNA and protein levels after various mitogenic and stress stimulations. DNAJC9 is mainly localized in cell nuclei under normal culture conditions, and transported into cytoplasm and plasma membrane upon heat shock stress through a non-classical and lipid-dependent pathway. DNAJC9 can interact with HSP70s and activate the ATPase activity of HSP70s, both of which are dependent on the J domain. Research has indicated that DNAJC9 is a novel cochaperone for HSP70s.1) Rose, J. Phd, et al. Mol. Med. (2010) In press : |
| Immunogen | This DNAJC9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human DNAJC9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DNAJC9 |
|---|---|
| Alternative Names | DnaJ homolog subfamily C member 9 DnaJ protein SB73 DNAJC9 |
| Molecular Weight(MW) | 30 kDa |
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
