Application
Immunohistochemistry of DNaseII in human spleen tissue with DNaseII antibody at 5 μg/mL.Application
Immunohistochemistry of DNase II in human spleen tissue with DNase II antibody at 5 μg/ml.Application
Immunofluorescence of DNase II in human spleen tissue with DNase II antibody at 5 μg/ml.Application
Western blot analysis of DNase II in human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide with DNase antibody II at 1:500 dilution.| Product Name | DNase II Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNase II Antibody: DNL, DNL2, DNASE2A, Deoxyribonuclease-2-alpha, Acid DNase, DNase II alpha |
| Product description | DNase II Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus. DNase II causes both chromatin condensation and DNA fragmentation. The genes encoding human, porcine, and murine DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation. DNase II is ubiquitously expressed in human tissues.1) Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8 |
| Immunogen | DNase II antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DNase II. The immunogen is located within the last 50 amino acids of DNase II. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| DNase II antibody can be used for detection of DNase II expression by Western blot at 0.5 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DNASE2 |
|---|---|
| Alternative Names | DNase II Antibody: DNL DNL2 DNASE2A Deoxyribonuclease-2-alpha Acid DNase DNase II alpha |
| Molecular Weight(MW) | Aprox: 40 kDa |
| Sequence Similarities | 3) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Immunohistochemistry of DNaseII in human spleen tissue with DNaseII antibody at 5 μg/mL.
Application
Immunohistochemistry of DNase II in human spleen tissue with DNase II antibody at 5 μg/ml.
Application
Immunofluorescence of DNase II in human spleen tissue with DNase II antibody at 5 μg/ml.
Application
Western blot analysis of DNase II in human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide with DNase antibody II at 1:500 dilution.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | DNase II antibody can be used for detection of DNase II expression by Western blot at 0.5 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DNase II antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DNase II Antibody is supplied in PBS containing 0.02% sodium azide. |
