Application
Immunofluorescence of DNM1L in HeLa cells with DNM1L antibody at 20 μg/mL.Application
Immunocytochemistry of DNM1L in HeLa cells with DNM1L antibody at 5 μg/mL.Application
Western blot analysis of DNM1L in HeLa cell lysate with DNM1L antibody at 1 μg/ml.| Product Name | DNM1L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNM1L Antibody: DLP1, DRP1, DVLP, EMPF, DYMPLE, HDYNIV, DLP1, Dynamin-1-like protein, Dynamin family member proline-rich carboxyl-terminal domain less |
| Product description | The Dynamin-1-like protein (DNM1L) is a member of the dynamin superfamily of GTPases (1). DNM1L mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis (2). Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF) (3).1) Shin HW, Shinotsuka C, Torii S, et al. Identification and subcellular localization of a novel mammalian dynamin-related protein homologous to yeast Vps1p and Dnm1p. J. Biochem. 1997; 122:525-30. |
| Immunogen | DNM1L antibody was raised against a 19 amino acid peptide near the center of human DNM1L. The immunogen is located within amino acids 530 - 580 of DNM1L. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| DNM1L antibody can be used for detection of DNM1L by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DNM1L |
|---|---|
| Alternative Names | DNM1L Antibody: DLP1 DRP1 DVLP EMPF DYMPLE HDYNIV DLP1 Dynamin-1-like protein Dynamin family member proline-rich carboxyl-terminal domain less |
| Molecular Weight(MW) | Predicted: 81 kDa Observed: 80 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | DNM1L antibody is human and mouse reactive. At least four isoforms of DNM1L are known to exist; this antibody will detect the two longest isoforms. |
Application
Immunofluorescence of DNM1L in HeLa cells with DNM1L antibody at 20 μg/mL.
Application
Immunocytochemistry of DNM1L in HeLa cells with DNM1L antibody at 5 μg/mL.
Application
Western blot analysis of DNM1L in HeLa cell lysate with DNM1L antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | DNM1L antibody can be used for detection of DNM1L by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DNM1L antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | DNM1L antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM277238