Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution + T47D whole cell lysates Lysates/proteins at 20 ug per lane.| Product Name | Dnmt3b Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, MHsaIIIB, DNMT3B |
| Product description | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3b is a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF)syndrome.1) Okano, M., et al., Cell 99(3):247-257 (1999). Yin, B., et al., Zhongguo Yi Xue Ke Xue Yuan Xue Bao 21(6):431-438 (1999). Okano, M., et al., Nat. Genet. 19(3):219-220 (1998). |
| Immunogen | This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-417 amino acids from human Dnmt3b. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | DNMT3B |
|---|---|
| Alternative Names | DNA (cytosine-5)-methyltransferase 3B Dnmt3b DNA methyltransferase HsaIIIB DNA MTase HsaIIIB MHsaIIIB DNMT3B |
| Molecular Weight(MW) | 96 kDa |
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution + T47D whole cell lysates Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
