Application
Immunohistochemistry of DOCK8 in human spleen tissue with DOCK8 antibody at 5 μg/mL.Application
Western blot analysis of DOCK8 in EL4 cell lysate with DOCK8 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | DOCK8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DOCK8 Antibody: MRD2, ZIR8, HEL-205, Dedicator of cytokinesis protein 8 |
| Product description | The Dedicator of cytokinesis protein 8 (DOCK8) is a member of the DOCK180 family of guanine nucleotide exchange factors (1). DOCK8 plays an essential role in humoral immune responses and is important in the proper formation of the B cell immunological synapse (reviewed in 2). Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome (3).1) Ruusala A and Aspenstrom P. Isolation and characterisation of DOCK8, a member of the DOCK180-related regulators of cell morphology. FEBS Lett. 2004; 572:159-66. |
| Immunogen | DOCK8 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human DOCK8. The immunogen is located within the last 50 amino acids of DOCK8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| DOCK8 antibody can be used for detection of DOCK8 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DOCK8 |
|---|---|
| Alternative Names | DOCK8 Antibody: MRD2 ZIR8 HEL-205 Dedicator of cytokinesis protein 8 |
| Molecular Weight(MW) | Predicted: 166, 220, 231 kDa Observed: 220, 230 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | DOCK8 antibody is human, mouse and rat reactive. Multiple isoforms of DOCK8 are known to exist. |
Application
Immunohistochemistry of DOCK8 in human spleen tissue with DOCK8 antibody at 5 μg/mL.
Application
Western blot analysis of DOCK8 in EL4 cell lysate with DOCK8 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | DOCK8 antibody can be used for detection of DOCK8 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DOCK8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | DOCK8 antibody is supplied in PBS containing 0.02% sodium azide. |
