Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | DOK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DOK2, p56DOK, p56dok-2 |
| Product description | DOK2 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210 (bcr/abl), a chimeric protein whose presence is associated with CML. DOK2 binds p120 (RasGAP) from CML cells.The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210 (bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells.1) Boulay, I., et al., (2005) J. Immunol. 175 (7), 4483-4489. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DOK2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DOK2 antibody can be used for detection of DOK2 by ELISA at 1:62500. DOK2 antibody can be used for detection of DOK2 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DOK2 |
|---|---|
| Alternative Names | DOK2 p56DOK p56dok-2 |
| Molecular Weight(MW) | 45 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | DOK2 antibody can be used for detection of DOK2 by ELISA at 1:62500. DOK2 antibody can be used for detection of DOK2 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DOK2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
