Application
Western blot analysis of DOK5 using rabbit polyclonal DOK5 Antibody (L291) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DOK5 gene.Application
Antibody is used in Western blot to detect DOK5 in mouse liver tissue lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | DOK5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Docking protein 5, Downstream of tyrosine kinase 5, Insulin receptor substrate 6, IRS-6, IRS6, DOK5, C20orf180 |
| Product description | DOK5 is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. In contrast to other DOK family proteins, this protein does not interact with RASGAP.1) Cai, D., et al., J. Biol. Chem. 278(28):25323-25330 (2003). |
| Immunogen | This DOK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-306 amino acids from the C-terminal region of human DOK5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | DOK5 |
|---|---|
| Alternative Names | Docking protein 5 Downstream of tyrosine kinase 5 Insulin receptor substrate 6 IRS-6 IRS6 DOK5 C20orf180 |
| Molecular Weight(MW) | 35 kDa |
Application
Western blot analysis of DOK5 using rabbit polyclonal DOK5 Antibody (L291) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DOK5 gene.
Application
Antibody is used in Western blot to detect DOK5 in mouse liver tissue lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
