Application
Dopamine Decarboxylase Antibody| Product Name | Dopamine Decarboxylase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DOPA decarboxylase, |
| Product description | Aromatic L-amino acid decarboxylase (AADC; DOPA decarboxylase; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin. It is also involved in the biosynthesis of trace amines.1) Haycock, J.W., Becker, L., Ang, L., Furukawa, Y., Hornykiewicz, O., and Kish, S.J., "Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum," J. Neurochem. 87 (2003) 574 - 585. |
| Immunogen | DOPA Decarboxylase polyclonal antibody was raised against a SDS denatured, recombinant bovine aromatic DDC expressed in E. coli and purified from inclusion bodies. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Applications include Dot Blots (DB) and Western Blots (WB). Recognizes DDC in rat adrenal medulla and caudate. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: |
|
| Species Reactivity | BovineRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DDC |
|---|---|
| Alternative Names | DOPA decarboxylase |
| Molecular Weight(MW) | 55 |
| Tissue Specificity | Dopamine Decarboxylase antibody is specific immunolabeling of the ~55k DDC protein in Western blots of rat adrenal medulla. |
Application
Dopamine Decarboxylase Antibody| Application Notes | Applications include Dot Blots (DB) and Western Blots (WB). Recognizes DDC in rat adrenal medulla and caudate. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
Data sheet for OM277290