Application
Immunohistochemistry of DPAGT1 in human kidney tissue with DPAGT1 antibody at 2.5 μg/mL.Application
Immunofluorescence of DPAGT1 in human kidney tissue with DPAGT1 antibody at 20 μg/mL.Application
Western blot analysis of DPAGT1 in mouse kidney tissue lysate with DPAGT1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | DPAGT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DPAGT1 Antibody: GPT, ALG7, DGPT, G1PT, UAGT, UGAT, CDG1J, DPAGT, CDG-Ij, CMSTA2, DPAGT2, D11S366, UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase, GlcNAc-1-P transferase |
| Product description | DPAGT1 Antibody: The UDP-N-acetylglucosamine-dolichyl-phosphate N-acetyl-glucosaminephosphotransferase (DPAGT1) is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. Mutations in this integral endoplasmic reticulum (ER) membrane protein enzyme belongs to the glycosyltransferase family 4 results in the congenital disorder of glycosylation type Ij with symptoms such as severe hypotonia, medically intractable seizures, mental retardation, microcephaly, and exotropia. Recent experiments have shown that DPAGT1 is a target of the Wnt/beta-catenin signaling pathway, with Wnt3a inducing higher DPAGT1 mRNA expression.1) Wu X, Rush JS, Karaoglu D, et al. Deficiency of UDP-GlcNAc:Dolichol Phosphate N-Acetylglucosamine-1 Phosphate Transferase (DPAGT1) causes a novel congenital disorder of glycosylation type Ij. Hum. Mutat. 2003; 22:144-50. |
| Immunogen | DPAGT1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human DPAGT1. The immunogen is located within amino acids 30 - 80 of DPAGT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| DPAGT1 antibody can be used for detection of DPAGT1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DPAGT1 |
|---|---|
| Alternative Names | DPAGT1 Antibody: GPT ALG7 DGPT G1PT UAGT UGAT CDG1J DPAGT CDG-Ij CMSTA2 DPAGT2 D11S366 UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase GlcNAc-1-P transferase |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | At least four isoforms of DPAGT1 are known to exist; this antibody will recognize the two longest isoforms. DPAGT1 antibody is predicted to not cross-react with UHRF1BP1. |
Application
Immunohistochemistry of DPAGT1 in human kidney tissue with DPAGT1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of DPAGT1 in human kidney tissue with DPAGT1 antibody at 20 μg/mL.
Application
Western blot analysis of DPAGT1 in mouse kidney tissue lysate with DPAGT1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | DPAGT1 antibody can be used for detection of DPAGT1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DPAGT1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DPAGT1 Antibody is supplied in PBS containing 0.02% sodium azide. |
