Application
Antibody used in WB on Human MCF7 at 1 ug/ml.Application
Antibody used in WB on Human HT1080 at 1 ug/ml.Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.Application
Antibody used in WB on Human NCI-H226 at 1 ug/ml.| Product Name | DPF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DPF3, CERD4, FLJ14079, BAF45C |
| Product description | DPF3 is a muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). DPF3 specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development.1) Hoyal, C.R., (er) J Carcinog 4, 13 (2005). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPF3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DPF3 antibody can be used for detection of DPF3 by ELISA at 1:12500. DPF3 antibody can be used for detection of DPF3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DPF3 |
|---|---|
| Alternative Names | DPF3 CERD4 FLJ14079 BAF45C |
| Molecular Weight(MW) | 40 kDa |
Application
Antibody used in WB on Human MCF7 at 1 ug/ml.
Application
Antibody used in WB on Human HT1080 at 1 ug/ml.
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.
Application
Antibody used in WB on Human NCI-H226 at 1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | DPF3 antibody can be used for detection of DPF3 by ELISA at 1:12500. DPF3 antibody can be used for detection of DPF3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DPF3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
