Application
Immunohistochemistry of DPF3 in human brain tissue with DPF3 antibody at 5 μg/mL.Application
Western blot analysis of DPF3 in mouse brain tissue lysate with DPF3 antibody at (A) 1 and (B) 2 μg/ml.Application
Immunofluorescence of DPF3 in human brain tissue with DPF3 antibody at 20 μg/mL.| Product Name | DPF3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DPF3 Antibody: CERD4, BAF45C, CERD4, Zinc finger protein DPF3, BRG1-associated factor 45C |
| Product description | The DPF3 protein, also known as Cerd4, is a member of the d4 gene family of transcription modulators that also includes DPF1/Neud4 and DPF2/Requiem (1). DPF3 has been shown to be a epigenetic key factor for heart and muscle development and can bind to methylated and acetylated lysine residues of histone 3 and 4, suggesting that DPF3 may play a role in recruiting chromatin remodeling complexes to acetylated histones (2). Two isoforms of DPF3, DPF3a and DPF3b, are required as transcriptional co-activators in SWI/SNF complex-dependent activation of the NF-kappaB RelA/p50 heterodimer (3).1) Ninkina NN, Mertsalov IB, Kulikova DA, et al. Cerd4, third member of the d4 gene family: expression and organization of genomic locus. Mamm. Genome 2001; 12:862-6. |
| Immunogen | DPF3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DPF3. The immunogen is located within amino acids 220 - 270 of DPF3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| DPF3 antibody can be used for detection of DPF3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DPF3 |
|---|---|
| Alternative Names | DPF3 Antibody: CERD4 BAF45C CERD4 Zinc finger protein DPF3 BRG1-associated factor 45C |
| Molecular Weight(MW) | Predicted: 39 kDa Observed: 50 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | DPF3 antibody is human and mouse reactive. Multiple isoforms of DPF3 are known to exist. |
Application
Immunohistochemistry of DPF3 in human brain tissue with DPF3 antibody at 5 μg/mL.
Application
Western blot analysis of DPF3 in mouse brain tissue lysate with DPF3 antibody at (A) 1 and (B) 2 μg/ml.
Application
Immunofluorescence of DPF3 in human brain tissue with DPF3 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | DPF3 antibody can be used for detection of DPF3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DPF3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | DPF3 antibody is supplied in PBS containing 0.02% sodium azide. |
