Application
Antibody used in IHC on Human Adult Liver at 1:100.Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Product Name | DPH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DPH1, DPH2L, DPH2L1, FLJ33211, OVCA1 |
| Product description | Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]). Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).1) Liu, S., (2004) Mol. Cell. Biol. 24 (21), 9487-9497. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPH1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| DPH1 antibody can be used for detection of DPH1 by ELISA at 1:312500. DPH1 antibody can be used for detection of DPH1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DPH1 |
|---|---|
| Alternative Names | DPH1 DPH2L DPH2L1 FLJ33211 OVCA1 |
| Molecular Weight(MW) | 49 kDa |
Application
Antibody used in IHC on Human Adult Liver at 1:100.
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | DPH1 antibody can be used for detection of DPH1 by ELISA at 1:312500. DPH1 antibody can be used for detection of DPH1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store DPH1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
