Application
Western blot analysis in A375 cell line lysates (35ug/lane).| Product Name | DPH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Diphthamide biosynthesis protein 1, DPH1 homolog, HsDph1, Diphthamide biosynthesis protein 2 homolog-like 1, DPH-like 1, DPH2-like 1, Diphthamide biosynthesis protein 2-like, Ovarian cancer-associated gene 1 protein, DPH1, DPH2L, DPH2L1, OVCA1 |
| Product description | Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM].1) Liu, S., et al. Mol. Cell. Biol. 24(21):9487-9497(2004) |
| Immunogen | This DPH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 384-412 amino acids from the C-terminal region of human DPH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DPH1 |
|---|---|
| Alternative Names | Diphthamide biosynthesis protein 1 DPH1 homolog HsDph1 Diphthamide biosynthesis protein 2 homolog-like 1 DPH-like 1 DPH2-like 1 Diphthamide biosynthesis protein 2-like Ovarian cancer-associated gene 1 protein DPH1 DPH2L DPH2L1 OVCA1 |
| Molecular Weight(MW) | 49 kDa |
Application
Western blot analysis in A375 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
