Application
Western blot analysis of DRAGON (RGMB) Antibody in K562 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with DRAGON (RGMB) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: mouse brain lysate Lane 3: mouse cerebellum lysate Lane 4: NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | DRAGON Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RGM domain family member B, DRG11-responsive axonal guidance and outgrowth of neurite, DRAGON, RGMB |
| Product description | Repulsive Guidance Molecule B (RGMB) is primarily expressed in the central nervous system. RGM A and B both appear at every level of the developing neural axis, where they colocalize to a large extent in the mantle layer, although only RGMA appears in the neuroepithelium, and only RGMB in the peripheral nervous system. During development, both RGMA and B appear also in lung and in limb cartilage, while RGMB has additional expression domains in pancreas.1) Schnichels,S., Gene Expr. Patterns(1), 1-11 (2007) |
| Immunogen | This DRAGON (RGMB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-406 amino acids from the C-terminal region of human DRAGON (RGMB). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | RGMB |
|---|---|
| Alternative Names | RGM domain family member B DRG11-responsive axonal guidance and outgrowth of neurite DRAGON RGMB |
| Molecular Weight(MW) | 48 kDa |
Application
Western blot analysis of DRAGON (RGMB) Antibody in K562 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with DRAGON (RGMB) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: mouse brain lysate Lane 3: mouse cerebellum lysate Lane 4: NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
