Application
Immunocytochemistry of DRAK2 in Jurkat cells with DRAK2 antibody at 10 μg/mL.Application
Western blot analysis of DRAK2 in Raji cell lysate with DRAK2 antibody at (A) 1 and B (2) μg/mL.| Product Name | DRAK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DRAK2 Antibody: DRAK2, DRAK2, DAP kinase-related apoptosis-inducing protein kinase 2 |
| Product description | DRAK2 Antibody: Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases). DRAKs contain an N-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues.1) Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine kinases related to death-associated protein kinase that trigger apoptosis. J Biol Chem 1998;273:29066-71 (RD1299) |
| Immunogen | DRAK2 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human DRAK2. The immunogen is located within the last 50 amino acids of DRAK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| DRAK2 antibody can be used for detection of DRAK2 by Western blot 0.5 μg/mL. An approximately 45 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STK17B |
|---|---|
| Alternative Names | DRAK2 Antibody: DRAK2 DRAK2 DAP kinase-related apoptosis-inducing protein kinase 2 |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
| Tissue Specificity | It has no cross responses to DAP or ZIP kinases. The approximately 70 kDa band is probably non-related to DRAK2 although it is peptide blockable. |
Application
Immunocytochemistry of DRAK2 in Jurkat cells with DRAK2 antibody at 10 μg/mL.
Application
Western blot analysis of DRAK2 in Raji cell lysate with DRAK2 antibody at (A) 1 and B (2) μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | DRAK2 antibody can be used for detection of DRAK2 by Western blot 0.5 μg/mL. An approximately 45 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DRAK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DRAK2 Antibody is supplied in PBS containing 0.02% sodium azide. |
