Application
Immunohistochemistry of DTX4 in human spleen tissue with DTX4 antibody at 5 μg/ml.Application
Immunofluorescence of DTX4 in human spleen tissue with DTX4 antibody at 20 μg/ml.Application
Western blot analysis of DTX4 in HeLa cell lysate with DTX4 antibody at 1 μg/ml.| Product Name | DTX4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Deltex 4 E3 ubiquitin ligase, RNF155 |
| Product description | The Deltex 4 E3 ubiquitin ligase (DTX4) is a member of a protein family that is involved in Notch signaling and neurogenesis (1). Recent studies have shown that DTX4 is also associated with the innate immune response (2). Following the activation of type I interferon signaling, the Nod-like receptor protein 4 (NLRP4) recruits DTX4 for Lys48-linked polyubiquitination and degradation of the kinase TBK1 (NAK), thereby maintaining immune homeostasis during antiviral innate immunity (2).1) Kishi N, Tang Z, Maeda Y, et al. Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis. Int. J. Dev. Neurosci. 2001; 19:21-35. |
| Immunogen | DTX4 antibody was raised against a 19 amino acid peptide near the center of human DTX4. The immunogen is located within amino acids 230 - 280 of DTX4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| DTX4 antibody can be used for detection of DTX4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DTX4 |
|---|---|
| Alternative Names | Deltex 4 E3 ubiquitin ligase RNF155 |
| Molecular Weight(MW) | Predicted: 68 kDa Observed: 68 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | DTX4 antibody is human and mouse reactive. At least two isoforms of DTX4 are known to exist; this antibody will detect both isoforms. DTX4 antibody is predicted to not cross-react with other DTX protein family members. |
Application
Immunohistochemistry of DTX4 in human spleen tissue with DTX4 antibody at 5 μg/ml.
Application
Immunofluorescence of DTX4 in human spleen tissue with DTX4 antibody at 20 μg/ml.
Application
Western blot analysis of DTX4 in HeLa cell lysate with DTX4 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | DTX4 antibody can be used for detection of DTX4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DTX4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | DTX4 antibody is supplied in PBS containing 0.02% sodium azide. |
