Application
Western blot analysis of DUSP14 using rabbit polyclonal DUSP14 Antibody (D183) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DUSP14 gene.Application
Western blot analysis of anti-DUSP14 Pab in mouse kidney tissue lysate (35ug/lane).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | DUSP14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dual specificity protein phosphatase 14, MKP-1-like protein tyrosine phosphatase, MKP-L, Mitogen-activated protein kinase phosphatase 6, MAP kinase phosphatase 6, MKP-6, DUSP14, MKP6 |
| Product description | DUSP14 is involved in the inactivation of MAP kinases. This proein dephosphorylates ERK, JNK and p38 MAP-kinases. In addition to antigen recognition by the T-cell receptor, T-cell activation requires a second signal from a costimulatory receptor, such as CD28, which interacts with B7-1 (CD80) and B7-2 (CD86) ligands on antigen-presenting cells. CD28 costimulation induces transcription of interleukin-2 and stabilizes newly synthesized IL2 through the activation of mitogen-activated protein kinases (MAPKs), such as ERK and JNK, and the subsequent creation of AP1 transcription factor. DUSP14 is a negative regulator of CD28 signaling.1) Marti, F., et al., J. Immunol. 166(1):197-206 (2001). |
| Immunogen | This DUSP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-198 amino acids from the C-terminal region of human DUSP14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | DUSP14 |
|---|---|
| Alternative Names | Dual specificity protein phosphatase 14 MKP-1-like protein tyrosine phosphatase MKP-L Mitogen-activated protein kinase phosphatase 6 MAP kinase phosphatase 6 MKP-6 DUSP14 MKP6 |
| Molecular Weight(MW) | 22 kDa |
Application
Western blot analysis of DUSP14 using rabbit polyclonal DUSP14 Antibody (D183) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DUSP14 gene.
Application
Western blot analysis of anti-DUSP14 Pab in mouse kidney tissue lysate (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
