Application
Immunocytochemistry of DYRK2 in 293 cells with DYRK2 antibody at 10 μg/mL.Application
Immunofluorescence of DYRK2 in 293 cells with DYRK2 antibody at 20 μg/mL.Application
Western blot analysis of DYRK2 in 293 cell lysate with DYRK2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | DYRK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DYRK2 Antibody: Dual specificity tyrosine-phosphorylation-regulated kinase 2 |
| Product description | DYRK2 Antibody: DYRK2 is a member of the dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family that is thought to be involved in cellular proliferation and apoptosis. Under normal conditions, nuclear but not cytoplasmic DYRK2 is ubiquitinated by MDM2, leading to its constitutive degradation. However, upon exposure to genotoxic stress, ATM phosphorylates DYRK2, leading to its dissociation from MDM2 and its phosphorylation of p53, thereby inducing apoptosis. Recent evidence also suggests that DYRK2 may serve as a scaffold that facilitates assembly of an E3 ubiquitin ligase.1) Becker W, Weber Y, Wetzel K, et al. Sequence characteristics, subcellular localization, and substrate specificity of DRYK-related kinases, a novel family of dual specificity protein kinases. J. Biol. Chem. 1998; 273:25893-902. |
| Immunogen | DYRK2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DYRK2. The immunogen is located within amino acids 520 - 570 of DYRK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| DYRK2 antibody can be used for detection of DYRK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DYRK2 |
|---|---|
| Alternative Names | DYRK2 Antibody: Dual specificity tyrosine-phosphorylation-regulated kinase 2 |
| Sequence Similarities | 2) Cat. No. 17-010 - 293 Cell Slide |
| Tissue Specificity | Two isoforms of DYRK2 are known to exist; this antibody will recognize both isoforms. DYRK2 antibody will not cross-react with other DYRK family members. |
Application
Immunocytochemistry of DYRK2 in 293 cells with DYRK2 antibody at 10 μg/mL.
Application
Immunofluorescence of DYRK2 in 293 cells with DYRK2 antibody at 20 μg/mL.
Application
Western blot analysis of DYRK2 in 293 cell lysate with DYRK2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | DYRK2 antibody can be used for detection of DYRK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | DYRK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | DYRK2 Antibody is supplied in PBS containing 0.02% sodium azide. |
