Application
Western blot analysis of lysed extracts from 3T3 cells using E2F-1 Antibody .| Product Name | E2F 1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RBP3, E2F-1, RBAP1, RBBP3, RAF, RBP3 |
| Product description | Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. |
| Immunogen | E2F-1 antibody was raised against a peptide sequence around aa.430~434 (G-D-L-T-P) derived from Human E2F-1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | E2F1 |
|---|---|
| Alternative Names | RBP3 E2F-1 RBAP1 RBBP3 RAF RBP3 |
| Molecular Weight(MW) | 70 kDa |
| Tissue Specificity | This antibody detects endogenous levels of total E2F-1 protein. |
| Entrez Gene | 1869 |
|---|---|
| Protein Accession | NP_005216.1 |
Application
Western blot analysis of lysed extracts from 3T3 cells using E2F-1 Antibody .| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
