Application
Antibody used in WB on Human Daudi cells at 0.2-1 ug/ml.| Product Name | E2F1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E2F1, RBP3, E2F-1, RBAP1, RBBP3 |
| Product description | The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.1) Unoki, M., et al., (2004) Oncogene 23 (46), 7601-7610. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| E2F1 antibody can be used for detection of E2F1 by ELISA at 1:312500. E2F1 antibody can be used for detection of E2F1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | E2F1 |
|---|---|
| Alternative Names | E2F1 RBP3 E2F-1 RBAP1 RBBP3 |
| Molecular Weight(MW) | 47 kDa |
Application
Antibody used in WB on Human Daudi cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | E2F1 antibody can be used for detection of E2F1 by ELISA at 1:312500. E2F1 antibody can be used for detection of E2F1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store E2F1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
