Application
Western Blot at 1:2000 dilution + Hela whole cell lysates Lysates/proteins at 20 ug per lane.Application
Western blot analysis of E2F1 Antibody (H357) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293T cell line lysate.Application
Western Blot at 1:1000 dilution Lane 1: NIH-3T3 whole cell lysates Lane 2: A431 whole cell lysates Lysates/proteins at 20 ug per lane.| Product Name | E2F1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1, E2F1, RBBP3 |
| Product description | E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another two members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.1) O'Donnell, K.A., et al., Nature 435(7043):839-843 (2005). Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005). Joshi, B., et al., Oncogene 24(13):2204-2217 (2005). Saberwal, G., et al., Int. J. Hematol. 80(2):146-154 (2004). Chaussepied, M., et al., Mol. Cell 16(5):831-837 (2004). |
| Immunogen | This E2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 amino acids from human E2F1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | E2F1 |
|---|---|
| Alternative Names | Transcription factor E2F1 E2F-1 PBR3 Retinoblastoma-associated protein 1 RBAP-1 Retinoblastoma-binding protein 3 RBBP-3 pRB-binding protein E2F-1 E2F1 RBBP3 |
| Molecular Weight(MW) | 47 kDa |
Application
Western Blot at 1:2000 dilution + Hela whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of E2F1 Antibody (H357) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293T cell line lysate.
Application
Western Blot at 1:1000 dilution Lane 1: NIH-3T3 whole cell lysates Lane 2: A431 whole cell lysates Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
