E2F7 is a E2F family member. It can block the E2F-dependent activation of a subset of E2F target genes as well as mitigate cellular proliferation of mouse embryo fibroblasts1) de (2003) J. Biol. Chem. 278 (43), 42041-42049.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse E2f7.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
E2f7 antibody can be used for detection of E2f7 by ELISA at 1:62500. E2f7 antibody can be used for detection of E2f7 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.
Positive Control
1) SP2/0 Cell Lysate
Application Notes
E2f7 antibody can be used for detection of E2f7 by ELISA at 1:62500. E2f7 antibody can be used for detection of E2f7 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store E2f7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.