EB1 family proteins are evolutionarily conserved proteins that bind microtubule plus-ends and centrosomes and regulate the dynamics and organization of microtubules. Human EB1 family proteins, which include EB1, EBF3, and RP1, also associate with the tumor suppressor protein adenomatous polyposis coli (APC) and p150glued, a component of the dynactin complex.1) Ota, T., et al., (2004) Nat. Genet. 36 (1), 40-45.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EBF3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
EBF3 antibody can be used for detection of EBF3 by ELISA at 1:62500. EBF3 antibody can be used for detection of EBF3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
EBF3 antibody can be used for detection of EBF3 by ELISA at 1:62500. EBF3 antibody can be used for detection of EBF3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store EBF3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt