Application
Immunohistochemistry of EBI3 in human spleen tissue with EBI3 antibody at 2.5 μg/mL.Application
Immunofluorescence of EBI3 in Human Spleen cells with EBI3 antibody at 20 μg/mL.Application
Western blot analysis of EBI3 in human tonsil tissue lysate with EBI3 antibody at 2 μg/mL.| Product Name | EBI3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EBI3 Antibody: IL27B, IL-27B, IL27B, Interleukin-27 subunit beta, Epstein-Barr virus-induced gene 3 protein, IL-27 subunit beta |
| Product description | EBI3 Antibody: EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35. Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.1) Pfanz S, Timans JC, Cheung J et al. IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naïve CD4(+) T cells. Immunity2002; 16:779-90. |
| Immunogen | EBI3 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human EBI3. The immunogen is located within the last 50 amino acids of EBI3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| EBI3 antibody can be used for the detection of EBI3 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EBI3 |
|---|---|
| Alternative Names | EBI3 Antibody: IL27B IL-27B IL27B Interleukin-27 subunit beta Epstein-Barr virus-induced gene 3 protein IL-27 subunit beta |
| Molecular Weight(MW) | Predicted: 25 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Immunohistochemistry of EBI3 in human spleen tissue with EBI3 antibody at 2.5 μg/mL.
Application
Immunofluorescence of EBI3 in Human Spleen cells with EBI3 antibody at 20 μg/mL.
Application
Western blot analysis of EBI3 in human tonsil tissue lysate with EBI3 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1315 - Human Tonsil Tissue Lysate |
|---|---|
| Application Notes | EBI3 antibody can be used for the detection of EBI3 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | EBI3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | EBI3 Antibody is supplied in PBS containing 0.02% sodium azide. |
