Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).Application
ECAT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | ECAT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KHDC3-like protein, ES cell-associated transcript 1 protein, KHDC3L, C6orf221, ECAT1 |
| Product description | 1) Pierre, A., et al. Genomics 90(5):583-594(2007) Mitsui, K., et al. Cell 113(5):631-642(2003) |
| Immunogen | This ECAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-48 amino acids from the N-terminal region of human ECAT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KHDC3L |
|---|---|
| Alternative Names | KHDC3-like protein ES cell-associated transcript 1 protein KHDC3L C6orf221 ECAT1 |
| Molecular Weight(MW) | 24 kDa |
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).
Application
ECAT1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
