Application
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ECE-1 Antibody in A2058 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with ECE-1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | ECE-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Endothelin-converting enzyme 1, ECE-1, ECE1 |
| Product description | ECE-1 is involved in proteolytic processing of endothelin precursors to biologically active peptides.1) Cottrell,G.S., et.al., J. Biol. Chem. 284 (33), 22411-22425 (2009) |
| Immunogen | This ECE-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 528-556 amino acids from the C-terminal region of human ECE-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ECE1 |
|---|---|
| Alternative Names | Endothelin-converting enzyme 1 ECE-1 ECE1 |
| Molecular Weight(MW) | 87 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ECE-1 Antibody in A2058 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with ECE-1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
