Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Product Name | ECH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial, 533-, ECH1 |
| Product description | This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.1) Olsen, J.V., et al. Cell 127(3):635-648(2006) |
| Immunogen | This ECH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-250 amino acids from the C-terminal region of human ECH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ECH1 |
|---|---|
| Alternative Names | Delta(3 5)-Delta(2 4)-dienoyl-CoA isomerase mitochondrial 533- ECH1 |
| Molecular Weight(MW) | 36 kDa |
Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
