Application
Western blot analysis of ECRG4 in HeLa cell lysate with ECRG4 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunocytochemistry of ECRG4 in HeLa cells with ECRG4 antibody at 5 μg/mL.| Product Name | ECRG4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ECRG4 Antibody: ECRG4, ECRG4, UNQ761/PRO1508, Augurin, Esophageal cancer-related gene 4 protein |
| Product description | ECRG4 Antibody: The esophageal cancer-related gene 4 (ECRG4), also known as Augurin, was identified through differential display screening between esophageal cancer and normal tissue. Hypermethylation of CpG sites in the ECRG4 promoter correlate with ECRG4 downregulation in numerous esophageal squamous cell carcinoma tissues.. In transfected cells, overexpression of ECRG4 induced G1 arrest, dephosphorylation of Rb and decreased expression of cyclins D1 and D3. Increased expression of ECRG4 in aged mouse brain is observed compared with young mouse brain. The increased ECRG4 level was accompanied by elevated senescence-associated acidic beta-galactosidase activity, suggesting that ECRG4 is involved in cellular differentiation and senescence.1) Su T, Liu H, and Lu S. Cloning and identification of cDNA fragments related to human esophageal cancer. Zhonghua Zhong Liu Za Zhi 1998; 20:254-7. |
| Immunogen | ECRG4 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human ECRG4. The immunogen is located within the last 50 amino acids of ECRG4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| ECRG4 antibody can be used for detection of ECRG4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C2orf40 |
|---|---|
| Alternative Names | ECRG4 Antibody: ECRG4 ECRG4 UNQ761/PRO1508 Augurin Esophageal cancer-related gene 4 protein |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | ECRG4 antibody is predicted to not cross-react with other ECRG family members. |
Application
Western blot analysis of ECRG4 in HeLa cell lysate with ECRG4 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunocytochemistry of ECRG4 in HeLa cells with ECRG4 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | ECRG4 antibody can be used for detection of ECRG4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ECRG4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ECRG4 Antibody is supplied in PBS containing 0.02% sodium azide. |
