Application
ECT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung squamous carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in 293 cell line lysates (35ug/lane).| Product Name | ECT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein ECT2, Epithelial cell-transforming sequence 2 oncogene, ECT2 |
| Product description | The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.1) Justilien, V., et al. Oncogene 28(41):3597-3607(2009) |
| Immunogen | This ECT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 597-625 amino acids from the Central region of human ECT2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ECT2 |
|---|---|
| Alternative Names | Protein ECT2 Epithelial cell-transforming sequence 2 oncogene ECT2 |
| Molecular Weight(MW) | 104 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
ECT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung squamous carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in 293 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
