WB
Various lysates were subjected to SDS PAGE followed by western blot with ECT2 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using ECT2 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using ECT2 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed A549 cells using ECT2 antibody at dilution of 1:400 and 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).IP
IP result of anti-ECT2 (IP:4ug; Detection:1:6000) with K-562 cells lysate 1640 ug.FC
1x10^6 A549 cells were intracellularly stained with 0.25 ug ECT2 Polyclonal antibody and 488-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.| Product Name | ECT2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ECT2 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:5000-1:50000 IHC:1:500 ICC/IF:1:200-1:800 IP: 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate FC:0.25 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ECT2 |
|---|---|
| Gene Synonyms | ARHGEF31 |
| Gene Full Name | epithelial cell transforming 2 |
| Gene Summary | The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017] |
| Alternative Names | Epithelial cell-transforming sequence 2 oncogene Protein ECT2 |
| Molecular Weight(MW) | 100 kDa |
| Cellular Localization | Extracellular matrix,Secreted. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with ECT2 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using ECT2 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using ECT2 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed A549 cells using ECT2 antibody at dilution of 1:400 and 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IP
IP result of anti-ECT2 (IP:4ug; Detection:1:6000) with K-562 cells lysate 1640 ug.
FC
1x10^6 A549 cells were intracellularly stained with 0.25 ug ECT2 Polyclonal antibody and 488-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit.| Application Notes | WB:1:5000-1:50000 IHC:1:500 ICC/IF:1:200-1:800 IP: 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate FC:0.25 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
