WB
Western Blot, Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate, All lanes:ECT2 antibody at 2.7μg/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
IHC image of ECT2 Antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.IP
Immunoprecipitating ECT2 in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of ECT2 Antibody in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000), Lane 2: ECT2 Antibody (6μg) + Hela whole cell lysate (1mg), Lane 3: Hela whole cell lysate (20μg).| Product Name | ECT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Protein ECT2 protein (283-453AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:5000 IHC:1:500-1:1000 IP:1:200-1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | ECT2 |
|---|---|
| Gene Synonyms | ARHGEF31 |
| Gene Full Name | epithelial cell transforming 2 |
| Gene Summary | The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017] |
| Molecular Weight(MW) | 104/100/109/101kDa(Observed MW 104kDa) |
| Cellular Localization | Nucleus. Cytoplasm. |
WB
Western Blot, Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate, All lanes:ECT2 antibody at 2.7μg/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
IHC image of ECT2 Antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IP
Immunoprecipitating ECT2 in Hela whole cell lysate, Lane 1: Rabbit control IgG instead of ECT2 Antibody in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000), Lane 2: ECT2 Antibody (6μg) + Hela whole cell lysate (1mg), Lane 3: Hela whole cell lysate (20μg).| Application Notes | WB:1:500-1:5000 IHC:1:500-1:1000 IP:1:200-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
