Application
Immunohistochemistry of EDA1 in mouse kidney tissue with EDA1 antibody at 5 μg/ml.Application
Immunofluorescence of EDA1 in mouse kidney tissue with EDA1 antibody at 20 μg/ml.Application
Western blot analysis of EDA1 in mouse kidney tissue lysate with EDA1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | EDA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ectodysplasin A, Ectodermal dysplasia protein, ED1, ED1-A1, ED1-A2, EDA1, EDA2, EDA protein, HED, ODT1, STHAGX1, XHED, XLHED |
| Product description | Ectodysplasin A (EDA1) is a member of the TNF-related ligand family involved in the early epithelial-mesenchymal interaction that regulates ectodermal appendage formation (1). It is a trimeric type II membrane protein that co-localizes with cytoskeletal structures at the lateral and apical surfaces of cells and can be expressed as eight alternatively spliced isoforms in hair follicles and in the epidermis of adult skin (2,3). EDAs are required during development, and loss or mutation of EDA1 results in a group of developmental disorders identified as ectodermal dysplasia type 1 (4,5).1) Kere J, Srivastava AK, Montonen O. X-linked anhidrotic (hypohidrotic) ectodermal dysplasia is caused by mutation in a novel transmembrane protein. Nat. Genet. 1996; 13:409-16. |
| Immunogen | EDA1 antibody was raised against an 19 amino acid peptide near the center of human EDA1. The immunogen is located within amino acids 130 - 180 of EDA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| EDA1 antibody can be used for detection of EDA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EDA |
|---|---|
| Alternative Names | Ectodysplasin A Ectodermal dysplasia protein ED1 ED1-A1 ED1-A2 EDA1 EDA2 EDA protein HED ODT1 STHAGX1 XHED XLHED |
| Molecular Weight(MW) | Predicted: 16, 43 kDa Observed: 39 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (89%) |
| Tissue Specificity | EDA1 antibody is human, mouse and rat reactive. Multiple isoforms of EDA1 are known to exist. |
Application
Immunohistochemistry of EDA1 in mouse kidney tissue with EDA1 antibody at 5 μg/ml.
Application
Immunofluorescence of EDA1 in mouse kidney tissue with EDA1 antibody at 20 μg/ml.
Application
Western blot analysis of EDA1 in mouse kidney tissue lysate with EDA1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | EDA1 antibody can be used for detection of EDA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | EDA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | EDA1 antibody is supplied in PBS containing 0.02% sodium azide. |
