Application
Confocal immunofluorescent analysis of EDN1 Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis in ZR-75-1,U251,MDA-MB453,A549 cell line lysates (35ug/lane).Application
EDN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | EDN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Endothelin-1, Preproendothelin-1, PPET1, Endothelin-1, ET-1, Big endothelin-1, EDN1 |
| Product description | The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.1) Gonsalves, C., et al. J. Immunol. 185(10):6253-6264(2010) |
| Immunogen | This EDN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-203 amino acids from the C-terminal region of human EDN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EDN1 |
|---|---|
| Alternative Names | Endothelin-1 Preproendothelin-1 PPET1 Endothelin-1 ET-1 Big endothelin-1 EDN1 |
| Molecular Weight(MW) | 24 kDa |
Application
Confocal immunofluorescent analysis of EDN1 Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis in ZR-75-1,U251,MDA-MB453,A549 cell line lysates (35ug/lane).
Application
EDN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
