Application
Western blot analysis of lysates from Hela, K562 cell line (from left to right), using EEF1B2 Antibody at 1:1000 at each lane.Application
Immunohistochemical analysis of paraffin-embedded H. brain section using EEF1B2 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Fluorescent image of Hela cells stained with EEF1B2 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).Application
Flow cytometric analysis of Hela cells using EEF1B2 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.| Product Name | EEF1B2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Elongation factor 1-beta, EF-1-beta, EEF1B2, EEF1B, EF1B |
| Product description | EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.1) Sanders J.,et al.Nucleic Acids Res. 19:4551-4551(1991). |
| Immunogen | This EEF1B2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 54-86 amino acids from the Central region of human EEF1B2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For FACS starting dilution is: 1:25 For IF starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EEF1B2 |
|---|---|
| Alternative Names | Elongation factor 1-beta EF-1-beta EEF1B2 EEF1B EF1B |
| Molecular Weight(MW) | 25 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Western blot analysis of lysates from Hela, K562 cell line (from left to right), using EEF1B2 Antibody at 1:1000 at each lane.
Application
Immunohistochemical analysis of paraffin-embedded H. brain section using EEF1B2 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Fluorescent image of Hela cells stained with EEF1B2 Antibody . Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Application
Flow cytometric analysis of Hela cells using EEF1B2 Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.| Application Notes | For FACS starting dilution is: 1:25 For IF starting dilution is: 1:25 For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
